| 摘要 |
Compositions for estimating protease activity comprise (a) a protein, (b) a dye indicator exhibiting protein error at pH1-2,8 and (c) an acid buffer providing said pH. The composition may be a solution or a bibulous carrier impregnated with a solution, the protein (preferably albumin, haemoglobin, mucin or casein) comprising 0,01-1,0% of the solution and the dye (preferably Orange IV, Metanil yellow, Thymol blue or Bromophenol blue) comprising 0,0005-0,005% of the solution. The acid in the buffer may be hydrochloric, citric or hexamic. Proteases to be estimated include pepsin, uropepsin, trypsin, chymotrypsin, ficin and papain. The composition may alternatively be a powder or tablet containing 1-10% protein, 0,1-1% dye and a buffer, which when dissolved forms a solution as above defined. On adding the composition to a protease-containing liquid, the colour changes as the protein is digested and the colour at a given time or the time to reach a given colour is indicative of the protease activity. Specification 826,066 is referred to.
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