CELL LINE CULTURABLE WITHOUT AMINAL-DERIVED COMPONENT, METHOD FOR ESTABLISHMENT THEREOF, METHOD FOR PRODUCTION OF VIRUS USING THE CELL LINE AND METHOD FOR PRODUCTION OF VACCINE

摘要

A cell line which is derived from MDCK cells (canine kidney-derived cells) and is culturable in the absence of any animal-derived component. The cell line can be established by a method for cell line establishment comprising the steps of acclimatizing MDCK cells to a serum-free culture medium containing a cell growth factor and culturing the cells using a culture medium which comprises RPMI 1640 medium and soybean-derived peptone but contains no animal-derived component, thereby producing a cell line culturable without any animal-derived component.