METHOD OF DETECTING MICROORGANISM AND BACTERIOLYTIC REAGENT

摘要

It is intended to provide a method of detecting a microorganism which comprises the extraction step of contacting microbial cells with a bacteriolytic reagent and thus extracting intracellular ATP from the microbial cells, the amplification step of amplifying the intracellular ATP thus extracted, and the light emission step of contacting the intracellular ATP with luciferin and luciferase for light emission, wherein the bacteriolytic reagent contains a lytic enzyme, a nonionic surfactant and a chelating agent. Thus, a method of detecting a microorganism, whereby a microorganism even in an extremely small amount can be sufficiently detected, and a bacteriolytic reagent are provided.