发明名称 |
Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
摘要 |
The present invention relates to the detection of nucleic acid sequence differences using coupled ligase detection reaction and polymerase chain reaction. One aspect of the present invention involves use of a ligase detection reaction coupled to a polymerase chain reaction. Another aspect of the present invention relates to the use of a primary polymerase chain reaction coupled to a secondary polymerase chain reaction coupled to a ligase detection reaction. A third aspect of the present invention involves a primary polymerase chain reaction coupled to a secondary polymerase chain reaction. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence differences. |
申请公布号 |
US7097980(B2) |
申请公布日期 |
2006.08.29 |
申请号 |
US20040852289 |
申请日期 |
2004.05.24 |
申请人 |
REGENTS OF THE UNIVERSITY OF MINNESOTA |
发明人 |
BARANY FRANCIS;LUBIN MATTHEW;BARANY GEORGE;HAMMER ROBERT P.;BELGRADER PHILLIP |
分类号 |
C12N15/09;C12Q1/68;C12P19/34 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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