METHOD FOR MEASURING SPINORPHIN

摘要

The present invention aims at providing a method for measuring spinorphin in a sample collected from a living body accurately with high sensitivity. The present invention provides a method for measuring spinorphin in a sample collected from a living body, comprising the steps of: (A) mixing a sample collected from a living body with trichloroacetic acid to give a solution phase and subjecting said solution phase and a solvent to a reverse phase column to elute spinorphin with said solvent; (B) contacting the spinorphin eluted in the step (A) with spinorphin immobilized on a carrier and a spinorphin antibody; (C) removing the spinorphin antibody that has not been bound to the spinorphin immobilized on the carrier; (D) contacting the spinorphin antibody bound to the spinorphin immobilized on the carrier obtained in the step (C) with a labeled secondary antibody that specifically binds to said spinorphin antibody so as to bind the spinorphin antibody bound to the spinorphin immobilized on the carrier and the labeled secondary antibody; and (E) measuring the amount of label in the labeled secondary antibody bound to the spinorphin antibody.