FLUORESCENCE CORRELATION MICROSCOPY WITH REAL-TIME ALIGNMENT READOUT
摘要
<p>This invention relates to a confocal fluorescence correlation microscope with real-time alignment read out. With this instrument it is possible to do confocal imaging together with the particle size determination at a chosen location in the specimen. In particular, this invention relates to a detector module with a fixed aperture and detection electronics that can be conveniently connected to an existing confocal or multiphoton microscope, near the base of the objective lens of the microscope. This detector splits a part of the signal and uses it to generate a spot on the confocal image. This shows the spot where an FCS measurement can be carried out, and the same signal can then be used to perform a fluorescence correlation measurement after parking the excitation beam of the confocal to that spot. No alignment step is necessary for obtaining the measurement.</p>
申请公布号
WO2006087727(A1)
申请公布日期
2006.08.24
申请号
WO2005IN00015
申请日期
2005.02.15
申请人
TATA INSTITUTE OF FUNDAMENTAL RESEARCH;MAITI, SUDIPTA;KAUSHALYA, SANJEEV, KUMAR;GARAI, KANCHAN;BALAJI, JAYAPRAKASH