发明名称 |
METHOD OF DETECTION OF CLASSICAL SWINE FEVER |
摘要 |
The invention relates to a multiplex real-time RT-PCR assay with a heterologous internal control system (i.e., EGFP-RNA) for the simple and fast diagnosis of classical swine fever virus (CSFV). Primers and FAM-labeled TaqMan probes, specific for CSFV were selected by analyzing the consensus sequence of the 5 '-non translated region of various CSFV strains. For determining the analytical sensitivity an in vitro transcript (T7-PC3Alf) of the 5' NTR was constructed and tested. Furthermore, a primer - probe system for the detection of the internal control sequence was established, and a multiplex assay using CSF-System 1 and the IC real-time PCR could be performed as a one-tube assay without loss of sensitivity or specificity. |
申请公布号 |
WO2006086377(A2) |
申请公布日期 |
2006.08.17 |
申请号 |
WO2006US04271 |
申请日期 |
2006.02.08 |
申请人 |
IDEXX LABORATORIES;HOFFMANN, BERND;DEPNER, KLAUS;BEER, MARTIN |
发明人 |
HOFFMANN, BERND;DEPNER, KLAUS;BEER, MARTIN |
分类号 |
C12Q1/70;C07H21/04;C07J17/00;C12Q1/68 |
主分类号 |
C12Q1/70 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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