发明名称 |
Single chain antibody and use thereof |
摘要 |
The present invention provides a single chain antibody that retains its original specific binding activity with an antigen, and a labeled single chain antibody in which a labeling substance is bound to the single chain antibody. Specifically, the labeled single chain antibody of the present invention can be produced by linking a labeling substance to a linker part of a single chain antibody. The antibody is produced using a wheat embryo-derived cell-free protein synthesis system, and production is carried out in a low reductive state that allows an intramolecular disulfide bond to be retained. Further, bonding the antibody to a solid phase via the labeling substance enables production of an immobilized single chain antibody as well as a method for analyzing an antigen-antibody reaction using the immobilized single chain antibody.
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申请公布号 |
US2006172344(A1) |
申请公布日期 |
2006.08.03 |
申请号 |
US20050522000 |
申请日期 |
2005.02.23 |
申请人 |
ENDO YAETA;KAWASAKI TAKAYASU;SAWASAKI TATSUYA |
发明人 |
ENDO YAETA;KAWASAKI TAKAYASU;SAWASAKI TATSUYA |
分类号 |
G01N33/53;C07H21/04;C07K16/00;C07K16/12;C07K16/46;C12P21/06 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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