发明名称 |
High throughput assay for identification of gene expression modifiers |
摘要 |
The present invention provides a method for screening of a large number of compounds for their ability to modulate the expression of genes. The method uses gene trap technology and comprises the steps of transfecting a population of cells with a gene-trap vector, sorting cells according to their level of fluorescence, distributing sorted cells into pools and expanding the pools to obtain a sufficient number of cells representing each trapped gene to permit distinction of the effect of a test compound over controls, exposing the cells to the test compounds and identifying compounds which alter the fluorescence distribution pattern of cells using FACS analysis.
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申请公布号 |
US7083919(B2) |
申请公布日期 |
2006.08.01 |
申请号 |
US20020139420 |
申请日期 |
2002.05.06 |
申请人 |
THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK |
发明人 |
PRUITT STEVEN C.;HANGAUER DAVID G.;STEWART CARLETON C.;MIELNICKI LAWRENCE MARK |
分类号 |
G01N33/50;C12N15/09;C12N15/10;C12Q1/06;C12Q1/68;G01N21/78;G01N33/15;G01N33/543 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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