发明名称 |
Recovery of plasmids in an aqueous two-phase system |
摘要 |
The present invention is a method for the purification of plasmid DNA comprising to provide a composition comprising a first polymer having inverse solubility characteristics and a second polymer immiscible in the first polymer; contacting said solution with an aqueous solution comprising plasmid DNA; providing phase separation and isolating the aqueous phase; and increasing the temperature of the isolated phase to a temperature above the cloud point of the first polymer and below the temperature where plasmid DNA is degraded and subsequently isolating the aqueous phase so formed. The invention also encompasses a kit for purification of plasmid DNA as described above.
|
申请公布号 |
US2006166349(A1) |
申请公布日期 |
2006.07.27 |
申请号 |
US20050526089 |
申请日期 |
2005.02.23 |
申请人 |
KEPKA CECILIA;RHODIN JENNY;TJERNELD FOLKE |
发明人 |
KEPKA CECILIA;RHODIN JENNY;TJERNELD FOLKE |
分类号 |
C12N15/09;C12N15/63;C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|