COMPOSITIONS AND METHODS FOR SYNTHESIZING, PURIFYING AND DETECTING BIOMOLECULES

摘要

Compositions and methods that can reduce or eliminate purification and detection difficulties related to SlyD polypeptides are disclosed. The compositions include cells that lack or contain. a reduced amount of SlyD, cells that contain SlyD mutated to, reduce or eliminate its ability to bind biarsenical reagents; anti-SlyD antibodies, and kits containing the same. The disclosed compositions can be used, e.g., to purify and detect recombinant polypeptides having polyhistidine or polycysteine tags.