发明名称 |
RNA INTERFERENCE THAT BLOCKS EXPRESSION OF PRO-APOPTOTIC PROTEINS POTENTIATES IMMUNITY INDUCED BY DNA AND TRANSFECTED DENDRITIC CELL VACCINES |
摘要 |
An immunotherapeutic strategy is disclosed that combines antigen-encoding DN A vaccine compositions combined with siRNA directed to pro-apoptotic genes, primarily Bak and Bax, the products of which are known to lead to apoptotic death. Gene gun delivery (particle bombardment) of siRNA specific for Bak and/or Bax to antigen-expressing DCs prolongs the lives of such DCs and lead to enhanced generation of antigen-specific CD8+T cell-mediated immune responses in vivo. Similarly, antigen-loaded DC~s transfected with siRNA targeting Bak and/or Bax serve as improved immunogens and tumor immunotherapeutic agents. |
申请公布号 |
CA2594040(A1) |
申请公布日期 |
2006.07.13 |
申请号 |
CA20052594040 |
申请日期 |
2005.12.30 |
申请人 |
THE JOHNS HOPKINS UNIVERSITY |
发明人 |
HUNG, CHIEN-FU;WU, TZYY-CHOU |
分类号 |
A61K48/00;C12N15/11;C12N15/113 |
主分类号 |
A61K48/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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