发明名称 |
METHOD OF INDUCING DIFFERENTIATION OF SKELETAL MUSCLE CELL |
摘要 |
<p>A method of inducing differentiation from a bone marrow stromal cell to a skeletal muscle cell effectively, simply and reproducibly, comprising the steps of :(a) adding one or more substances selected from the group consisting of a cyclic AMP elevation functioning agent, a cyclic AMP analogue and a cell differentiation survival functioning factor to a culture of a bone marrow stromal cell (note that the bone marrow stromal cell is not treated with a demethylating agent) and performing cultivation; (b) obtaining a myoblast culture by introducing Notch gene and/or a Notch signaling related gene into the cell obtained in the step (a) and performing cultivation (note that the cultivation does not include co-cultivation of a cell into which a gene has been introduced and a cell into which a gene has not been introduced); and (c) adding a Notch ligand to the myoblast culture obtained in the step (b) and performing cultivation.</p> |
申请公布号 |
WO2006068225(A1) |
申请公布日期 |
2006.06.29 |
申请号 |
WO2005JP23598 |
申请日期 |
2005.12.22 |
申请人 |
KYOTO UNIVERSITY;DEZAWA, MARI;NABESHIMA, YO-ICHI;HOSHINO, MIKIO |
发明人 |
DEZAWA, MARI;NABESHIMA, YO-ICHI;HOSHINO, MIKIO |
分类号 |
C12N5/10;A61K35/28;A61K35/34;A61P21/00;A61P21/04;A61P41/00;A61P43/00;C12N5/077;C12N15/09 |
主分类号 |
C12N5/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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