| 摘要 |
<p>A method for removing extraneous nucleotides in a cloned coding sequence using a type IIs endonuclease, the method comprising introducing a linker that comprises at least one recognition site for a Type IIs restriction endonuclease to the ORF, cloning the ORF into a suitable vector, and removing the extraneous nucleotides from the vector with a RE IIs digestion. Also provided are a vector, a kit and oligonucloetides suitable for the invention.</p> |
