A method of reading an assay using low resolution detection

摘要

A method of reading an assay includes illuminating a substrate (100). The substrate includes a sensitive region (102). The method also includes detecting whether the substrate (100) was exposed to an analyte of interest. The detecting includes using low resolution detection and a priori data about the sensitive region (102) to determine whether a characteristic of a first portion of the illumination returned from the sensitive region (102) differs from a characteristic of a second portion of the illumination returned from the substrate outside the sensitive region (103).