Method and apparatus of quantitative assays

摘要

A sample testing apparatus ( 100 ) for use in measuring an analyte in a sample ( 105 ) is disclosed. The apparatus includes a sample well ( 104 ) for receiving the sample. A solid analyte binding agent ( 160 ) is disposed in the sample well. The apparatus further includes a mixing device ( 156 ) for mixing the sample with the solid analyte binding agent and a valve ( 129 ). The valve is configurable between a closed position for impeding flow of the sample from the sample well and an open position for releasing the sample from the sample well. The apparatus additionally includes a membrane ( 118 ) for receiving the sample once released from the sample well, the membrane having a capture reagent ( 164 ) adapted to interact with at least one of the analyte, the analyte binding agent, or a complex thereof, to aid in determining the amount of the analyte in the sample.