发明名称 |
PROTEINEXPRESSION IN BACULOVIRUS-VEKTOR EXPRESSIONSYSTEMEN |
摘要 |
The invention relates to a method to increase protein expression in baculo vector virus expression systems. The invention provides a method to produce a recombinant protein in insect-cell culture which comprises selecting a recombinant baculovirus expressing said protein, growing insect cells in growth medium in a culture vessel with a sufficient volume to contain at least 2 liters and infecting the cells with an inoculum of at least one baculovirus at a cell density of 1 x 10<5> to 5 x 10<6> cells/ml with an m.o.i of <0.01. The invention furthermore provides a method to produce recombinant pestivirus E2 or E<rns> protein or fragments thereof in insect cell culture characterised by a final concentration of said protein (fragments) in the growth medium at harvest of at least 100 mu g/ml. The invention furthermore provides a method to produce recombinant follicle stimulating hormone, alpha -units and/or beta -units and complexes and fragments thereof, at a concentration in the growth medium at harvest of at least 15 mu g/ml. |
申请公布号 |
DE69834346(D1) |
申请公布日期 |
2006.06.01 |
申请号 |
DE1998634346 |
申请日期 |
1998.12.17 |
申请人 |
STICHTING INSTITUUT VOOR DIERHOUDERIJ EN DIERGEZONDHEID;BAYER AG |
发明人 |
KRETZDORN, DIETMAR;VAN DE WIEL, FRANCISCUS;DE SMIT, JOHANNES;MOORMANN, JACOBUS;HAMANN, KEES |
分类号 |
C12N15/09;C12N15/63;A61K39/00;A61K39/12;A61K39/187;A61P31/12;A61P31/14;C07K14/18;C07K14/185;C12N7/00;C12N15/40;C12N15/866;C12P21/00;C12P21/02 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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