TERMINAL-PHOSPHATE-LABELED NUCLEOTIDES WITH NEW LINKERS
摘要
The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.
申请公布号
WO2004072238(A3)
申请公布日期
2006.05.18
申请号
WO2004US03284
申请日期
2004.02.05
申请人
AMERSHAM BIOSCIENCES CORP;KUMAR, SHIV;MCDOUGALL, MARK;SOOD, ANUP;NELSON, JOHN;FULLER, CARL;MACKLIN, JOHN;MITSIS, PAUL
发明人
KUMAR, SHIV;MCDOUGALL, MARK;SOOD, ANUP;NELSON, JOHN;FULLER, CARL;MACKLIN, JOHN;MITSIS, PAUL