| 摘要 |
<p>It is intended to provide an easy-to-use and highly reliable assay method, whereby the number of detection procedure steps can be decreased in a method of testing a sample with the use of a flow-through type membrane immunoassay method, and a kit to be used in this method. Namely, a simplified flow-through type membrane immunoassay method for detecting a subject in a test sample with the use of an assay apparatus provided with a membrane to which a scavenger antibody or antigen for capturing the subject to be detected is bound, characterized by comprising filtering the test sample by using a filter and then dropping, allowing the scavenger antibody or antigen on the membrane to capture the subject to be detected, dropping an enzyme-labeling reagent, which comprises an enzyme-labeled substance capable of reacting with the detection subject, onto the membrane carrying the detection subject having been captured, and detecting the presence of the detection subject in the test sample without resorting to a washing step; and a kit for detecting the presence of a subject in a test sample.</p> |
