<p>It is intended to provide a primer having a promoter region added thereto; and a method of using the primer having a promoter region added thereto in RNA amplification, biological gene expression analysis and/or verification analysis. A primer is constructed by adding a promoter region to a random primer capable of binding to a part highly complementary to an RNA or a primer containing a base sequence complementary to a specific gene. Then, the obtained primer is employed in RNA amplification, biological gene expression analysis and/or verification analysis.</p>