A DIFFERENTIAL ALTERNATING FIELD ELECTROPHORESIS METHOD AND AN ELECTROPHORESIS SYSTEM THEREFOR

摘要

The invention describes use of a differential alternating field electrophoresis (DAFE) method where electrical fields in substantially opposing directions are applied to proteins for separation thereof. By varying the duration of the electrical fields, forward directional and inverse directional pulsing of the electrical fields creates an advancing- dislodging effect on the proteins. The advancing-dislodging effect of the DAFE method facilitates migration of the proteins through the separation gel and thereby results in improved separation of the proteins using conventional electrophoresis devices. This overcomes many of the limitations suffered by Sodium Doecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE).