| 摘要 |
<p>A method of screening a compound capable of binding to a transmembrane region of transmembrane enzyme to thereby inhibit any enzymatic activity thereof, characterized in that with the use of protein (a) having part or the whole of the amino acid sequence of the enzyme wherein a region including the activity center of the transmembrane enzyme and part or the whole of the transmembrane region are contained, optionally together with protein (b) having part of the amino acid sequence of the transmembrane enzyme wherein a region including the activity center is contained while part or the whole of the transmembrane region is lacking, there are measured the binding of each test compound to these proteins and the enzymatic activities of these particles. Further, there is provided a screening kit comprising the above proteins (a) and (b). Still further, there is provided a ß-secretase selective inhibitor comprising a substance capable of inhibiting the enzyme which binds to the transmembrane region of ß-secretase, specifically a preventive/therapeutic agent for Alzheimer disease, Down syndrome or senile memory disorders.</p> |
