A high content screening (HCS) assay for rapidly screening one or more compounds to determine functional response or pharmacological properties thereof, comprising: i) priming a cell or cell material with a sensor for a biological response; ii) contacting the compound(s) to be tested with the primed cell or cell material or contacting a cell or cell material which has been contacted with the compound(s) with the primed cell or cell material; iii) simultaneously or subsequently contacting with a fluorescent agonist or a fluorescent neutral antagonist wherein the binding of the fluorescent agonist or antagonist and its associated biological response are detected or monitored in the same cell and are distinct allowing separate readout.
申请公布号
WO2006032926(A2)
申请公布日期
2006.03.30
申请号
WO2005GB03709
申请日期
2005.09.26
申请人
THE UNIVERSITY OF NOTTINGHAM;HILL, STEVEN, JOHN;KELLAM, BARRIE;BRIDDON, STEPHEN, JOHN
发明人
HILL, STEVEN, JOHN;KELLAM, BARRIE;BRIDDON, STEPHEN, JOHN