| 摘要 |
To determine the presence of a specific nucleic acid sequence within a sample, a labelled substance, capable of binding the nucleic acid to be determined, or a labelling substance is added, the substance having the ability to label the nucleic acid or being representative for the nucleic acid. The nucleic acid is amplified, the presence of which is to be determined and the increase of the labelled substance and/or the effect initiated by the labelled substance due to the increase of this specific nucleic acid is determined. The signal increase and/or the effect against time is analysed using a model for determining a deviation from a linear curve.
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