Method of measuring amadori compound by light scattering

摘要

In order to quantitatively measure an Amadori compound by a simple optical method utilizing light scattering, a sample containing an Amadori compound which is stored in a cell (3) is irradiated with excitation light from an He-Ne laser unit (1) so that scattered light from the sample is received and separated into its spectral components for obtaining a light scattering spectrum, and a light scattering peak existing at 820 to 840 cm<-1>, 1655 to 1660 cm<-1>, 2000 to 2020 cm<-1>, 2080 to 2100 cm<-1>, 2460 to 2470 cm<-1> or 2530 to 2600 cm<-1> in shift wavenumber with respect to the excitation wavelength in the light scattering spectrum is detected by a detector (23). The saccharide concentration or the saccharification ratio of the Amadori compound is measured by a calibration curve through the peak intensity or the peak integral value of the light scattering peak.