| 发明名称 |
THERMOSTABLE LUCIFERASES FROM PHOTURIS PENNSYLVANICA AND PYROPHORUS PLAGIOPHTHALAMUS AND METHODS OF PRODUCTION |
| 摘要 |
<p>Luciferase enzymes with greatly increased thermostability, e.g., at least half lives of 2 hours at 50°C, cDNAs encoding the novel luciferases, and hosts transformed to express the luciferases, are disclosed. Methods of producing the luciferases include recursive mutagenesis. The luciferases are used in conventional methods, some employing kits.</p> |
| 申请公布号 |
EP1630231(A2) |
申请公布日期 |
2006.03.01 |
| 申请号 |
EP20050076634 |
申请日期 |
1999.12.22 |
| 申请人 |
PROMEGA CORPORATION |
发明人 |
WOOD, MONIKA G.;WOOD, KEITH V.;HALL, MARY P. |
| 分类号 |
C12N15/53;G01N21/78;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/02;C12N9/10;C12N9/12;C12N9/24;C12N9/38;C12N15/09;C12N15/10;C12Q1/02;C12Q1/66 |
主分类号 |
C12N15/53 |
| 代理机构 |
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