METHOD FOR PRODUCING ANTIGEN FOR PERFORMING SALMONELLA CARRIAGE DIAGNOSIS IN HENS

摘要

veterinary medicine. SUBSTANCE: method involves culturing Salmonella enterifides and Salmonella pullorum-gallinarum separately on hard nutrient media. The mature cultures are washed out with physiological salt solution. The outwashed material is collected in the same reservoir. The produced microbe suspension is inactivated in microwave stove. Ultra-high frequency radiation frequency is equal to 2450±509 MHz. The treatment lasts 1 to 5 min with the medium heated not higher than 80 C. After having been cooled, the microbe suspension is centrifuged. The cells are washed and stained. Antigen is produced which quality is not lower than that of pullorum erythrocyte antigen. EFFECT: enhanced effectiveness in sanitizing agricultural farms from salmonellosis; low cost of poultry examination. 3 cl, 2 tbl