STRAIN OF BACTERIUM BACILLUS SUBTILIS AS PRODUCER OF RESTRICTION ENDONUCLEASE Bisi

摘要

FIELD: biotechnology, biochemistry, enzymology, genetic engineering. ^ SUBSTANCE: invention relates to preparing new strain used for isolation of the new restriction endonuclease Bis I that can be used in detection of the modified DNA. The strain Bacillus subtilis 230 isolated from soil as result of search of producers of restriction endonucleases provides preparing the restriction endonuclease Bis I recognizing and cleaving both chain of DNA nucleotide sequence comprising at least one C5-methylcytosine base in the recognition site 5'-GCNGC-3'. ^ EFFECT: valuable properties of strain, new enzyme. ^ 1 tbl, 3 dwg, 2 ex