发明名称 |
Verfahren zur Herstellung von optisch aktivem 4-Halo-3-hydroxybuttersäureester |
摘要 |
The present invention provides a method for preparing (S)-4-halo-3-hydroxybutyric acid ester comprises asymmetric reduction of 4-halo-acetoacetic acid ester using beta -ketoacyl-acylcarrier protein reductase constituting Type II fatty acid synthase or acetoacetyl-CoA reductase constituting the poly- beta -hydroxy fatty acid synthesis system. beta -ketoacyl-acyl carrier protein reductase constituting Type II fatty acid synthase or acetoacetyl-CoA reductase constituting the poly- beta -hydroxy fatty acid synthesis system has a extremely high reducing activity as well as stereoseletivity for (S)-4-chloro-3-hydroxybutyric acid ester. In addition, the enzyme exhibits almost no oxidizing activity toward either configuration of ethyl 4-chloro-3-hydroxybutyrate, performing only the reducing reaction of ethyl 4-chloroacetoacetate. Therefore, this enzyme can be used to efficiently produce (S)-4-halo-3-hydroxybutyric acid ester. |
申请公布号 |
DE69920568(T2) |
申请公布日期 |
2006.02.16 |
申请号 |
DE1999620568T |
申请日期 |
1999.05.10 |
申请人 |
DAICEL CHEMICAL INDUSTRIES, LTD. |
发明人 |
YAMAMOTO, HIROAKI |
分类号 |
C12N15/09;C12N1/21;C12N9/02;C12N9/04;C12N15/00;C12P7/42;C12P7/62;C12R1/01;C12R1/125;C12R1/19 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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