VARIANTEN DER GALLENSÄURE-STIMULIERTEN LIPASE, DNA DIE DAFÜR KODIERT, UND TRANSGENE NICHT-HUMANE SÄUGETIERE

摘要

The invention discloses nucleic acids encoding variant Bile Salt Stimulated Lipase (BSSL; EC 3.1.1.1). The encoded variant BSSL enzymes maintain catalytic activity but contain fewer glycosylation sites that full-length BSSL. This reduced glycosylation facilitates purification and characterization of recombinant BSSL proteins.