发明名称 |
A METHOD FOR EFFICIENT TRANSFER OF HUMAN BLASTOCYST-DERIVED STEM CELLS (HBS CELLS) FROM A FEEDER-SUPPORTED TO A FEEDER-FREE CULTURE SYSTEM |
摘要 |
<p>A method for the transfer of human blastocyst-derived stem cells (hBS cells) to feeder-free culture system and propagation of the cells in such a feeder-free culture system, the method comprising the following steps of (a) transferring the balstocyst-derived stem cells from feeder to feeder free culture by mechanical treatment, (b) optionally, culturing the blastocyst-derived stem cells under feeder cell free growth conditions in a suitable growth medium and/or on a suitable support substrate, and (c) optionally passaging the blastocyst derived stem cell line every 3-10 days by enzymatic and/or mechanical treatment. The invention also relates to the application of hBS cells cultured under feeder free condition in medicine (e.g., myocardial regeneration) and screening and toxicity tests.</p> |
申请公布号 |
EP1625213(A2) |
申请公布日期 |
2006.02.15 |
申请号 |
EP20040731930 |
申请日期 |
2004.05.10 |
申请人 |
CELLARTIS AB |
发明人 |
ERIKSSON, PETER;KILMARE, EVA KARIN;TALLHEDEN, TOMMI;ENERBAECK, S. |
分类号 |
A01N1/02;A61K35/12;A61P9/00;A61P25/28;C12N5/0797;(IPC1-7):C12N5/08;A61K35/54 |
主分类号 |
A01N1/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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