| 摘要 |
A fully functional artificial organ pseudo-body and a preparation and culturing method thereof, relating to the interdisciplinary fields of biology, materials, and manufacturing. The pseudo-body comprises an outer skin layer (101) and an organ main body tissue area (102); the organ main body tissue area (102) comprises a growth area (103), a differentiation area (104), a jointing area (105), a branch arterial system (106), a branch nervous system (107) and a branch venous system (108); the branch arterial system (106), the branch nervous system (107), and the branch venous system (108) are distributed in the differentiation area (104), and with the outer growth area (103) and the middle jointing area (105) form a main body 3D framework structure. Hydrogels having different cells and growth factors are arranged on the 3D framework structure using techniques including droplet spray, infusion, and injection; synthetic polymers are attached to the outer side of the 3D framework structure using techniques including adhesion or spraying to form an appearance similar to that of various animal organs; and the organ pseudo-body is cultured using a specific culturing apparatus and method, such that the function and structure thereof mimics those of an animal organ, providing new possibilities for fields such as organ manufacturing and transplantation. |
