METHODS FOR ENHANCING PLANT TRANSFORMATION FREQUENCIES
摘要
The invention provides improved transformation methods. In particular the method provides increased transformation frequency, especially in recalcitra nt plants. The method comprises stably transforming a target cell with one or more vectors containing at least one polynucleotide of interest. The target cell has been previously modified to stimulate growth of the cell and has go ne through at least one cell division.
申请公布号
CA2414471(C)
申请公布日期
2006.02.07
申请号
CA20012414471
申请日期
2001.07.09
申请人
PIONEER HI-BRED INTERNATIONAL, INC.
发明人
CHURCH, LAURA A.;LOWE, KEITH S.;GORDON-KAMM, WILLIAM J.;HILL, PATREA M.;ROSS, MARGIT C.;BIDNEY, DENNIS L.;HOERSTER, GEORGE J.