METHOD OF PREPARING ARTIFICIAL GENE POPULATION AND ARTIFICIAL PROTEIN POPULATION BY RANDOM POLYMERIZATION OF MOTIF SEQUENCES OF MULTIPLE TYPES

摘要

In order to create an artificial functional gene or an artificial functional protein, it is intended to provide an artificial gene population obtained via the random polymerization of motif sequences of multiple types, an artificial protein population encoded by the above-described artificial gene population and a method of preparing the same. An artificial gene population obtained via the random polymerization of motif sequences of multiple types is prepared by constructing at least three types of DNA sequences each having a single-stranded DNA containing a sequence encoding an arbitrary motif sequence, transferring a complementary base sequence thereinto so as to allow the formation of a complementary base pair in part of the terminal sequences of the single-stranded DNA sequences with each other, and then treating a liquid reaction mixture, which is prepared by mixing the thus constructed single-stranded DNAs of multiple types at an arbitrary ratio, with DNA polymerase so that random polymerization proceeds among the DNAs encoding the motif sequences. By translating the artificial gene population and converting the same into artificial proteins, an artificial protein population encoded by the motif sequences of multiple types can be prepared.