| 摘要 |
FIELD: medicine. ^ SUBSTANCE: method involves sawing bone into 0.1-2.0 cm thick plates, washing them with 0.1 M phosphate buffer solution having pH equal to 5.8-6.0. The tissue is digested in activated 0.125-0.325% papain at 60°C during 24 h. The plates are washed with 5 water volumes at 60°C and treated at room temperature C during 10-24 h with alkaline solution of 0.4 pH, washed with running water, treated with 3% hydrogen peroxide during 4 h, degreased in mixture of ethanol: chloroform in 1:2 proportion changing solution for a new one not less than twice, decalcified in hydrochloric acid solution of 0.5-1.0 pH, washed with purified water and dried at room temperature, packed and sterilized using radiation. ^ EFFECT: high collagen quality; low antigen activity. |
