| 摘要 |
<p>The present invention relates to stable expression systems for production of a protein comprising: a) a host organism selected from the group consisting of the species of Acanthamoeba ; b) a DNA vector which comprises a selection marker gene which codes for a protein which, after transformation of the host organism, allows selection of positive transformants and is selected from the group consisting of antibiotic resistance genes or of genes which encode proteins which are capable of a color-forming reaction, where expression of the selection marker gene is controlled by at least one genetic regulatory element which is homologous in the host organism; and c) a DNA vector which comprises a heterologous gene for the expression of a heterologous protein, where the expression of the protein is controlled by at least one genetic regulatory element which is homologous to the host organism.</p> |
