MUTANT GLYCOPROTEIN NOT MODIFIED WITH ASPARAGINE-LINKED SUGAR CHAIN

摘要

To obtain a mutant protein having no N-linked sugar chain, which is bonded in an asparagine-linked glycoprotein under ordinary circumstance, and yet sustains the physiological activity of the glycoprotein before the transfer of the mutation, at least one of the amino acids contained in the amino acid sequence motives (I) and/or (II) in the polypeptide of the asparagine-linked glycoprotein is substituted into another amino acid: (I) Asn Xa1 Xa2 (II) Xa3 Val Gly Asn Xa1 Xa2. In the formulae (I) and (II), Xa1 represents an amino acid other than Pro, Xa2 represents Thr or Ser and Xa3 represents His or Asp.