发明名称 |
Dual expression vector system and screening methods |
摘要 |
The present invention features vectors that contain a promoter effective for expression in bacterial cells and a promoter effective for expression in insect cells. The dual promoter system allows use of the same vector in both host cell systems so that construction of only a single vector is needed to express a polynucleotide inserted at a downstream cloning site. In preferred embodiments the vector is used to derive a recombinant baculovirus that is used to infect host cells. In particular vectors the promoters are a baculovirus polh promoter and a T7lac promoter. In particular vectors the promoter effective for expression in bacteria is positioned between the promoter effective for expression in insect cells and a cloning site. The invention also features various high throughput screening methods.
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申请公布号 |
US2006019337(A1) |
申请公布日期 |
2006.01.26 |
申请号 |
US20050033774 |
申请日期 |
2005.01.12 |
申请人 |
CHAMBERS STEPHEN P |
发明人 |
CHAMBERS STEPHEN P. |
分类号 |
C12P21/06;C12N5/06;C12N15/70;C12N15/74;C12N15/86;C12N15/866 |
主分类号 |
C12P21/06 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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