METHOD OF SCREENING SUBSTANCE INTERACTING WITH ABC PROTEIN

摘要

The present invention provides the following: a measuring method for transporter activity of ABC protein that can measure not only small amount of samples with small data fluctuation and good reproducibility, but also many samples simultaneously and concisely; a screening method for substances that interact with ABC protein using said measuring method; and a kit for measuring transporter activity of ABC protein that can be used advantageously for those methods. Pre-incubation is carried out by adding the insect cellular membrane expressing human P-glycoprotein to the reactionbuffer solution including vanadic acid and [ 3 H] ATP. Following this, the test compound is added for reaction, after which reaction liquid is added to a glass filter of 96-well type, and by using the suction cleaning method, adsorption amount of [ 3 H] ADP into the membrane-expressing P-glycoprotein is measured through 1 step.