METHOD OF SCREENING TOXIN-NEUTRALIZING PEPTIDE, STX2 INHIBITORY PEPTIDE AND VERO TOXIN-NEUTRALIZING AGENT

摘要

A screening method comprising the following steps: (1) specifying a receptor-binding site by transferring a mutation; and (2) specifying a binding site-specific peptide motif based on an amino acid selection ration by comparing a peptide motif binding to a wild type subunit with a peptide motif binding to a mutant functionally lacking the above-described binding site by the peptide library method. Thus, a peptide inhibiting a toxin having a subunit structure in the receptor-binding part is screened. Accordingly, it is possible to provide an STX2 inhibitor which has an STX2 inhibitory peptide integrated into a molecular nucleus structure having three lysine (Lys) molecules bonded together via peptide bonds, can be easily synthesized and can inhibit Vero toxin.