发明名称 |
STATION ZUR NUKLEINSÄUREVERVIELFÄLTIGUNG FÜR WEGWERFTESTTRÄGER |
摘要 |
An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber. |
申请公布号 |
DE60018386(T2) |
申请公布日期 |
2005.12.29 |
申请号 |
DE2000618386T |
申请日期 |
2000.09.29 |
申请人 |
BIOMERIEUX, INC. |
发明人 |
KLUTTZ, BRIAN;MCKINLEY, A.;GENNARI, FABIO;GUY, MICHEL;COTTER, CHRISTOPHER;CATANZARITI, LUIGI;GRAZIANO, LOUIS;COLIN, BRUNO;PARIS, CECILE;DACHAUD, JACQUES |
分类号 |
C12N15/09;B01J19/00;B01J19/24;B01L3/00;B01L7/00;C12M;C12M1/00;C12M1/18;C12M1/34;C12M3/00;C12Q1/68;G01N21/00;G01N33/50;G01N35/02;G01N37/00 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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