发明名称 |
Detection of protein translocation by beta-galactosidase reporter fragment complementation |
摘要 |
Methods and compositions are provided for detecting molecular translocations, particularly protein translocations within and between subcellular copartments, using at least two components that exhibit a localization-dependent difference in complementation activity. In particular, alpha-complementing beta-galactosidase fragments are provided. These beta-galactosidase reporter fragments display significantly enhanced enzymatic activity when one fragment is localized in a membrane. Methods for carrying out no-wash ELISA assays based on the reporter component system are also provided.
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申请公布号 |
US2005287522(A1) |
申请公布日期 |
2005.12.29 |
申请号 |
US20050132764 |
申请日期 |
2005.05.18 |
申请人 |
BLAU HELEN M;WEHRMAN THOMAS S |
发明人 |
BLAU HELEN M.;WEHRMAN THOMAS S. |
分类号 |
C12N9/38;C12Q1/68;C12Q1/70;G01N33/50;G01N33/53;G01N33/537;G01N33/542;G01N33/543;(IPC1-7):C12Q1/70 |
主分类号 |
C12N9/38 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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