发明名称 |
Animal component free meningococcal polysaccharide fermentation and seedbank development |
摘要 |
Animal-free meninge fermentation media and process is developed based upon use of a chemically defined medium. To improve polysaccharide production, fed-batch fermentation is examined using different feed solutions and feeding strategies. A feed solution containing glucose, amino acids, and trace metal elements produces Group A polysaccharide at approximately 3 times the level observed with batch fermentation. This process is used successfully to produce polysaccharides of N. meningitidis serotypes A, C, Y and W-135 and is run reproducibly at the 20L scale and can be scaled to 400L or more.
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申请公布号 |
US2005287649(A1) |
申请公布日期 |
2005.12.29 |
申请号 |
US20050204562 |
申请日期 |
2005.08.16 |
申请人 |
EGEN RICHARD C;FORTIN LORI A;SUN WILLIE W Q |
发明人 |
EGEN RICHARD C.;FORTIN LORI A.;SUN WILLIE W.Q. |
分类号 |
C12N1/20;C12P19/04;(IPC1-7):C12P19/04 |
主分类号 |
C12N1/20 |
代理机构 |
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