METHOD FOR PREVENTING NONSPECIFIC ADSORPTION OF FLUORESCENT INTERCALATOR TO SOLID PHASE SURFACE, HYBRIDIZATION DETECTION METHOD USING THE METHOD, AND SUBSTRATE FOR BIOASSAY

摘要

PROBLEM TO BE SOLVED: To prevent effectively nonspecific adsorption of an intercalator onto the solid state surface. SOLUTION: The solid state surface f for detecting hybridization between a nucleic acid D for detection and a target nucleic acid T is covered with a material to be positively charged such as avidin or modified with a functional group to be positively charged resulting from a silane coupling agent, to thereby prevent nonspecific adsorption of the fluorescent intercalator I onto the solid state surface f. Thus this method is especially useful when detecting hybridization on the solid state surface f by using the fluorescent intercalator I. COPYRIGHT: (C)2006,JPO&NCIPI