Use of lif for tissue and cellular engineering

摘要

Use of of any of leukemia inhibitory factor (LIF), or its analogs, mimetics and/or products that stimulate expression of endogenous LIF (collectively (A)), for: (i) promoting in vitro multiplication of a population of human skin stem cells and/or epidermal progenitor cells, while keeping them undifferentiated; and/or (ii) maintaining and/or increasing their capacity to generate a multilayered epithelium. Independent claims are also included for the following: (1) method for producing a library or culture of human skin stem cells and/or epidermal progenitor cells that includes at least one step of multiplication of a human keratinocyte population in a culture medium that contains (A); (2) kit for process (1); (3) methods for preparing reconstructed epidermis or skin; and (4) kit for process (3). ACTIVITY : Dermatological; Vulnerary. No details of tests for these activities are given. MECHANISM OF ACTION : None given.