| 摘要 |
FIELD: biotechnology, preparative biochemistry, enzymes. ^ SUBSTANCE: invention relates to a method for preparing proteolytic enzymes and can be used medicine, veterinary science and other branches. Method involves milling (homogenization) of pancreas tissue in tap water without using buffer solutions, extraction of product, separation of inert proteins and lipids. Separation of inert proteins and lipids is carried out by addition of 1% acid solution of chitosan to homogenate, stirring and settling. Prepared mixture is filtered through belting-fabric, trypsin-containing filtrate is concentrated by ultrafiltration through hollow fibers and subjected for drying. Invention provides increasing yield of trypsin, to enhance its specific activity, to simplify the technological process in preparing trypsin and to reduce duration of the process, to enhance safety and ecological purity of manufacturing process. ^ EFFECT: improved preparing method. ^ 2 tbl, 5 ex |
