发明名称 |
Directed evolution of proteins |
摘要 |
Disclosed is a method of altering a nucleic acid such as RNA or DNA. The method comprises fragmenting a parent nucleic acid strand to generate nucleic acid fragments. At least a subset of the fragments are ligated to generate shuffled nucleic acid strands. A selected strand is identified from the shuffled nucleic acid strands for a criterion. The methods of the invention can also be used to diversify proteins.
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申请公布号 |
US2005260655(A1) |
申请公布日期 |
2005.11.24 |
申请号 |
US20050107335 |
申请日期 |
2005.04.15 |
申请人 |
PRESIDENT AND FELLOWS OF HARVARD COLLEGE |
发明人 |
LIU DAVID R.;BITTKER JOSHUA A.;LIU JANE M. |
分类号 |
C12N15/09;C12N15/10;C12N15/62;C12P21/06;C12Q1/68;(IPC1-7):C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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