NOVEL HIV-BASED RECOMBINANT VIRAL CLONES AND USE THEREOF IN ANALYTICAL METHODS

摘要

The invention relates to HIV-based recombinant viral clones having the general structure shown in the accompanying figure, which are the result of the following genetic manipulations: deletion of HIV fragments (e.g. the Nef gene) without loss of infective capacity; insertion of a gene that is not expressed in human cells; insertion of the LacZ gene; and introduction of restriction sites, in order to extract DNA fragments from the matrix provirus and substitute same with genes from the patients to be assessed. In addition, the invention relates to the application of said clones in analytical methods that are associated with AIDS.