| 摘要 |
<p>Process of capillary electrophoresis in free solution having an alkaline pH for analysis of samples (I) comprising protein components (having lipoprotein components), comprises introducing (I) into a capillary tube containing an analysis buffer (comprising an additive of anionic surfactant capable of hydrophobic interaction with lipoprotein components) and modifying the electrophoretic mobility of the buffer. Independent claims are also included for: (1) process of separation of protein components (comprising alpha 1-, alpha 2-, beta 1 globulin in a buffer and lipoproteins) in liquid (I) by capillary electrophoresis in free solution having an alkaline pH, comprising passing the sample (comprising the components) into a capillary containing the analysis buffer, which is capable of interacting with the lipoproteins comprising at least an additive (a compound comprising an (negatively charged) anionic center (having a pH higher than 9 and a hydrophobic part comprising at least 10-20C alkyl chain); (2) electrolyte composition for capillary electrophoresis comprising at least a buffer and an additive capable of interacting hydrophobically with the lipoproteins in a medium; (3) composition for capillary electrophoresis comprising the analysis buffer and the additive comprising a hydrophobic part (comprising optionally 10-24C alkyl chain (comprising a linear part of at least 10 carbon atoms and an anionic center comprising one or more sulfonates, carboxylate, sulfates or phosphates)); and (4) an analytic truss for protein components in (I) comprising at least an analysis buffer, anionic surfactant additive (capable of allowing migration of the lipoproteins outside the zones where they usually migrate, particularly outside the fraction zones (alpha 1, alpha 2 and beta 1) comprising a hydrophobic part such as 10-20C alkyl chain and an anionic part carrying a strong negative charge (having a pH higher than 9)), capillary washing solution, appropriate diluents and/or dilution brooches.</p> |
