| 摘要 |
A polypeptide (I) having a biological activity of an NAD- or NADP-dependent alcohol dehydrogenase, and comprising any one of 33 fully defined 128, 161, 70, 94, 92, 93, 120, 140, 138, 144, 73, 75, 79, 76, 72, 74, 62, 67 or 348 amino acids (SEQ ID Nos. 1-33) sequence given in specification, or an amino acid sequence having 55-99% identity to any one of (SEQ ID Nos. 1-33), is new. A polypeptide (I) having a biological activity of an NAD- or NADP-dependent alcohol dehydrogenase, and comprising any one of 33 fully defined 128, 161, 70, 94, 92, 93, 120, 140, 138, 144, 73, 75, 79, 76, 72, 74, 62, 67 or 348 amino acids (SEQ ID Nos. 1-33) sequence given in specification, an amino acid sequence having 55-99% identity to any one of (SEQ ID Nos. 1-33), or an amino acid sequence encoded by a DNA sequence having any one of 34 fully defined 488, 385, 486, 483, 210, 282, 276, 279, 360, 421, 414, 432, 220, 226, 237, 229, 216, 225, 222, 186, 231, 216, 201 or 1047 base pairs (SEQ ID Nos. 35-68) sequence given in specification. Independent claims are also included for the following: (1) a nucleic acid molecule (II) comprising a sequence encoding (I), or complementary to the sequence encoding (I); (2) a vector (III) comprising (II); (3) a nonhuman host (IV) which comprises (I), (II) or (III); (4) a reaction system (V) comprising an organic compound which is a substrate of a dehydrogenase, (I), (III), or (IV) and a cofactor for (I); (5) preparation of (I); (6) a ligand (VI) which specifically binds (I), where the ligand is neither a substrate or cofactor of (I) nor a product generated from (I); (7) a primer (VII), having any one of 46 sequences of (ADHM1-46) from 5'-3', as given in Table 1; (8) a primer pair (VIII), having specific sequences of (ADHM1-46) as in Table 1, with the first primer of the primer pair serving as a forward primer and the second primer of the primer pair serving as a reverse primer to amplify a DNA sequence; and (9) a kit comprising any one of (I)-(VIII). (ADHM1-46) AAAGCATGCGGCGTTTGYCAYACNGA (forward), GCTTCAGGGCCTGGTAGGBSGTSAYSCC (reverse), GCCCAAGCCGGTCGTAAYNCCRCANCC (forward), TGGCGGCCAGGCCSAYNSCNCC (reverse), TGCGGCATCTGCGGSWCSGA (forward), TGAACGGCAAACAGGCCNAYNGGNCC (reverse), CGCCACCGCCATCTGYGGBWSBGA (forward), GACGGCGAACAGGCCNAYNGGVCC (reverse), ACAACGTGGTCGTGTACGGNCCNTGGGG (forward), GATGGTCGGCTGGTAGCCNACRAARTCNA (reverse). |
